Optimization of conditions for increasing of saffron cell biomass and crocin production in stirred bioreactor
نویسندگان
چکیده
Bioreactors provide suitable conditions for the growth of cells and production secondary metabolites by regulating physical chemical factors. In this study, saffron cell crocin metabolite were evaluated in Erlenmeyer flask a stirred bioreactor. To optimize conditions, effect different concentrations 2-(N-morpholino) ethanesulfonic acid (MES) (0, 2.5 5 mM) as buffering agent sucrose (3%, 6% gradually 6%) addressed on flask. This aim was then pursued bioreactor through investigation aeration pH medium adjustment. The results first step experiment showed that Schenk Hildebrandt basal with naphthalene acetic (2 mg l−1) 6-benzylaminopurine (1 supplemented mM MES well gradual increment from 3 to caused highest biomass production. spectrophotometry measurement content 0.8 g−1 after 6 weeks. second part revealed bioreactor, constant (5.8) during period is limiting factor Aeration also found be an inhibiting crocin. that, if evaporated volume water constituted, it can effective increase around 2 folds. addition, total proliferated system under non-constant without could raised up dry weight, based HPLC determination. According concluded tested natural fluctuation condition proper biomass, replaced. However obtained at value not capable increasing Saffron, most expensive spice contains valuable compounds like Corm derived-cell containing produced higher scales cheaper price using culture
منابع مشابه
faciliting lexical access for the fluent production of speech
the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis,a one-way anova experimental design was carried out. 24 sednior students of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inter...
15 صفحه اولfacilitating lexical access for the fluent production of speech
the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis , a one- way anova experimental design was carried out. 24 senior student of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inte...
15 صفحه اولmolecular and physiological characterizations of floral scent production in rosa damascena mill.
گل محمدی (r. damascena mill.)مهمترین گونه ای است که در تولید گلاب، عطر و اسانس، در صنعت عطرسازی و دارویی کاربرد دارد. با وجود اهمیت عطر گل محمدی، پژوهش های مولکولی و بیوشیمیایی عطر گل این گونه هنوز در مراحل ابتدایی است. در این پژوهش، ویژگی های مولکولی و فیزیولوژیکی تولید عطر در گل محمدی در آزمایش های مختلف مورد بررسی قرار گرفتند. در آزمایش اول، پژوهشی به منظور بررسی تنوع شیمیایی اسانس میان 44 ت...
the effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)
cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Cell Tissue and Organ Culture
سال: 2022
ISSN: ['1573-5044', '0167-6857']
DOI: https://doi.org/10.1007/s11240-022-02233-4